anti stat5a (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
anti stat5a
Anti Stat5a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stat5a/Stat5a+Antibody/pm41898298-93-0-1
Average 95 stars, based on 499 article reviews
Anti Stat5a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stat5a/Stat5a+Antibody/pm41898298-93-0-1
Average 95 stars, based on 499 article reviews
anti stat5a - by Bioz Stars,
2026-10
95/100 stars
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Chromatin Immunoprecipitation:Article Title: Cell-specific and shared regulatory elements control a multigene locus active in mammary and salivary glands. Article Snippet: .. The following antibodies were used for ChIP-seq: Article Title: Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development Article Snippet: The chromatin was fragmented to 250–500 bp using sonicator 3000 (30 cycles; 20 s pulse/20 s rest, Misonix Sonicators), checked on DNA gel, and further lysed in RIPA buffer. .. One milligram of total protein was immunoprecipitated with Dynabeads Protein A (Novex) coated with ChIP-seq grade antibodies: Article Title: Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development. Article Snippet: The chromatin was fragmented to 250–500 bp using sonicator 3000 (30 cycles; 20 s pulse/20 s rest, Misonix Sonicators), checked on DNA gel, and further lysed in RIPA buffer. .. One milligram of total protein was immunoprecipitated with Dynabeads Protein A (Novex) coated with ChIP-seq grade antibodies: Article Title: STAT5B leukemic mutations, altering SH2 tyrosine 665, have opposing impacts on immune gene programs Article Snippet: .. The following antibodies were used for ChIP-seq: Article Title: STAT5B SH2 variants disrupt mammary enhancers and the stability of genetic programs during pregnancy Article Snippet: .. The following antibodies were used for ChIP-seq: Immunoprecipitation:Article Title: Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development Article Snippet: The chromatin was fragmented to 250–500 bp using sonicator 3000 (30 cycles; 20 s pulse/20 s rest, Misonix Sonicators), checked on DNA gel, and further lysed in RIPA buffer. .. One milligram of total protein was immunoprecipitated with Dynabeads Protein A (Novex) coated with ChIP-seq grade antibodies: Article Title: Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development. Article Snippet: The chromatin was fragmented to 250–500 bp using sonicator 3000 (30 cycles; 20 s pulse/20 s rest, Misonix Sonicators), checked on DNA gel, and further lysed in RIPA buffer. .. One milligram of total protein was immunoprecipitated with Dynabeads Protein A (Novex) coated with ChIP-seq grade antibodies: Expressing:Article Title: Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development Article Snippet: Antigen retrieval was performed by boiling the slides in Antigen Unmasking Solution (Vector Laboratories) for 10 min. Endogenous peroxidase activity was quenched by incubating the slides in freshly prepared 3% hydrogen peroxide for 10 min. After rinsing in water, non-specific binding was blocked by incubating the slides with 2.5% goat serum (Vector Laboratories) for 1 h at room temperature. .. The expression of STAT5A and STAT5B proteins was detected by overnight incubation with primary antibodies: Incubation:Article Title: Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development Article Snippet: Antigen retrieval was performed by boiling the slides in Antigen Unmasking Solution (Vector Laboratories) for 10 min. Endogenous peroxidase activity was quenched by incubating the slides in freshly prepared 3% hydrogen peroxide for 10 min. After rinsing in water, non-specific binding was blocked by incubating the slides with 2.5% goat serum (Vector Laboratories) for 1 h at room temperature. .. The expression of STAT5A and STAT5B proteins was detected by overnight incubation with primary antibodies: Article Title: Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary Development. Article Snippet: .. MACS2 [37] peak-finding algorithm was used to identify 1 3 Page 3 of 20 7 Journal of Mammary Gland Biology and Neoplasia (2025) 30:7 detected by overnight incubation with primary antibodies: Phospho-proteomics:Article Title: Capitalizing on paradoxical activation of the mitogen-activated protein kinase pathway for treatment of Imatinib-resistant mast cell leukemia. Article Snippet: DMSO was obtained from Carl Roth GmbH & Co. .. Pelleted cells were solubilized with 0.5% NP‐40% and 0.5% sodium deoxycholate in 4°C phosphorylation solubilization buffer.28 After normalizing for protein content, lysates were supplemented with Lämmli buffer, boiled for 5 min at 95°C and subjected to SDS‐PAGE and subsequent Western blot analysis as previously described.28 The following antibodies were used for detection of BRAF (F‐7, sc‐5284), CRAF (C‐12, sc‐133), KIT (M‐14, sc‐1494), Western Blot:Article Title: Capitalizing on paradoxical activation of the mitogen-activated protein kinase pathway for treatment of Imatinib-resistant mast cell leukemia. Article Snippet: DMSO was obtained from Carl Roth GmbH & Co. .. Pelleted cells were solubilized with 0.5% NP‐40% and 0.5% sodium deoxycholate in 4°C phosphorylation solubilization buffer.28 After normalizing for protein content, lysates were supplemented with Lämmli buffer, boiled for 5 min at 95°C and subjected to SDS‐PAGE and subsequent Western blot analysis as previously described.28 The following antibodies were used for detection of BRAF (F‐7, sc‐5284), CRAF (C‐12, sc‐133), KIT (M‐14, sc‐1494), |